Human Monocytes - CD14, CD16 - Ziegler-Heitbrock

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Amyloid precursor protein expression in circulating monocytes and brain macrophages from patients with HIV-associated cognitive impairment

Abstract

We examined amyloid precursor protein (APP) surface expression on circulating leukocytes and in brain tissues from normal individuals and HIV+ subjects with cognitive impairment. Most monocytes, and a subset of B-lymphocytes, expressed APP, while T-lymphocytes, granulocytes, and natural killer (NK) cells did not. CD14bright/CD16+ monocytes expressed the highest levels, and CD14dim/CD16+ cells were negative, suggesting a relationship with activation. Higher APP+ monocyte levels correlated with increased numbers of CD16+ monocytes, but not with the degree of cognitive impairment. Treatment of monocytes with M-CSF, but not LPS, upregulated APP expression. In the brain, APP appeared as axonal immunoreactivity and diffuse plaques, and APP+ perivascular macrophages were seen in cases with severe dementia. APP may facilitate monocyte entry into the brain.

Authors: Vehmas A, Lieu J, Pardo CA, McArthur JC, Gartner S
Journal: J Neuroimmunol., 157(1-2):99-110
Year: 2004
PubMed: Find in PubMed