Human Monocytes - CD14, CD16 - Ziegler-Heitbrock

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The importance of being "pure" neutrophils.

Abstract

To the Editor: The identification of polymorphonuclear neutrophils as cellular source of cytokines prompts researchers in the field to investigate whether the production of neutrophil-derived cytokines can be altered in human diseases.1 For such a purpose, we developed a simple procedure that allows the isolation of more than 99.6% pure neutrophils (here defined as Neu) from either whole blood or buffy coats.

Authors: Calzetti F, Tamassia N, Arruda-Silva F, Gasperini S, Cassatella MA.
Journal: J Allergy Clin Immunol. 2017 Jan;139(1):352-355.e6
Year: 2017
PubMed: Find in PubMed