Human Monocytes - CD14, CD16 - Ziegler-Heitbrock

Contact

Assay of pyrogens by interleukin-6 release from monocytic cell lines

Abstract

A novel in-vitro system has been developed for the detection and quantification of pyrogen in pharmaceutical products. The measured variable was evoked secretion of the pyrogenic cytokine interleukin-6 from MONO MAC 6 monocytic cells incubated with the product. The interleukin-6 was detected using a specific and sensitive ELISA developed for this purpose. The test system detected pyrogenic contamination in 3 batches of therapeutic human serum albumin which had caused adverse reactions in recipients. The contamination was not detected in conventional tests: the rabbit pyrogen test and the limulus amoebocyte lysate test.

Authors: Taktak, Y.S., Selkirk, S., Bristow, A.F., Carpenter, A., Ball, C., Rafferty, B., Poole, S.
Journal: J. Pharm. Pharmacol., 43: 578-582
Year: 1991
PubMed: Find in PubMed