Human Monocytes - CD14, CD16 - Ziegler-Heitbrock

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Mononuclear cell subpopulations in CSF and blood of children with bacterial meningitis.

Abstract

OBJECTIVES: To elucidate mononuclear cell subsets of the cerebrospinal fluid (CSF) in order to investigate the pathogenesis of bacterial meningitis (BM). METHODS: Mononuclear cell and lymphocyte subsets in CSF and peripheral blood from 10 children with acute-stage BM before treatment on the same day were measured by flow cytometry. The control subjects for the subsets of peripheral blood were 15 healthy children. RESULTS: The percentages of CD14+ monocytes/macrophages (median: 56.5%), activated CD14+CD16+ monocytes/macrophages (20.9%), and CD14+CD16+ cells among total CD14+ cells (37.9%) in the CSF were significantly higher than those in the blood of children with BM (p<0.01, p<0.01, and p<0.05, respectively), which were significantly higher than those of the controls (p<0.001, p<0.001, p<0.05, respectively). The percentages of CD3+ (77.3%), CD4+ (45.2%), and CD8+ T cells (32.6%) in the CSF were significantly higher than those in the blood of affected children (p<0.01, p<0.01, and p<0.05, respectively). The percentages of CD3+, CD4+, and CD8+ T cells in the blood of children with BM were significantly lower than those of controls (all p<0.001). The percentages of CD20+ B cells (6.9%) in the CSF were significantly lower than those in the blood of affected children (p<0.01), which were significantly higher than those of controls (p<0.001). CONCLUSION: The percentages of monocytes/macrophages and T cells in CSF were higher than those in blood in children with BM.

Authors: Ichiyama T, Kajimoto M, Matsushige T, Shiraishi M, Suzuki Y, Furukawa S.
Journal: J Infect. 58(1):28-31
Year: 2009
PubMed: Find in PubMed